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click ittm edu cell proliferation kit  (Thermo Fisher)


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    Thermo Fisher click ittm edu cell proliferation kit
    Click Ittm Edu Cell Proliferation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10500 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/click ittm edu cell proliferation kit/product/Thermo Fisher
    Average 99 stars, based on 10500 article reviews
    click ittm edu cell proliferation kit - by Bioz Stars, 2026-02
    99/100 stars

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    click-ittm edu cell proliferation kit (thermofisher c10337)  (Thermo Fisher)
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    Thermo Fisher click-ittm edu cell proliferation kit (thermofisher c10337)
    Edu was added during pre-OL stage (day 35-40, panel A ) or immature-to-mature OL stage (day 50-55, panel B ) of iPSC differentiation, concurrently with CHPG (30μM final concentration), CHPG+MPEP (20μM final concentration), or BDNF (10ng/ml final concentration) treatment. ( C-D ) On day 55, oligodendrocytes that proliferated were quantified by Edu detection and co-immunofluorescent staining with O4. CHPG significantly increased <t>proliferation</t> of OL lineage cells only when CHPG was given during pre-OL stage (day 35-40) (N = 3 independent experiments). The effect was reversed by MPEP. Scale bar = 25μm.
    Click Ittm Edu Cell Proliferation Kit (Thermofisher C10337), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Edu was added during pre-OL stage (day 35-40, panel A ) or immature-to-mature OL stage (day 50-55, panel B ) of iPSC differentiation, concurrently with CHPG (30μM final concentration), CHPG+MPEP (20μM final concentration), or BDNF (10ng/ml final concentration) treatment. ( C-D ) On day 55, oligodendrocytes that proliferated were quantified by Edu detection and co-immunofluorescent staining with O4. CHPG significantly increased proliferation of OL lineage cells only when CHPG was given during pre-OL stage (day 35-40) (N = 3 independent experiments). The effect was reversed by MPEP. Scale bar = 25μm.

    Journal: bioRxiv

    Article Title: The mGluR5 Agonist, CHPG, Enhances Numbers of Differentiated Human Oligodendrocytes

    doi: 10.1101/2025.05.31.656838

    Figure Lengend Snippet: Edu was added during pre-OL stage (day 35-40, panel A ) or immature-to-mature OL stage (day 50-55, panel B ) of iPSC differentiation, concurrently with CHPG (30μM final concentration), CHPG+MPEP (20μM final concentration), or BDNF (10ng/ml final concentration) treatment. ( C-D ) On day 55, oligodendrocytes that proliferated were quantified by Edu detection and co-immunofluorescent staining with O4. CHPG significantly increased proliferation of OL lineage cells only when CHPG was given during pre-OL stage (day 35-40) (N = 3 independent experiments). The effect was reversed by MPEP. Scale bar = 25μm.

    Article Snippet: Olig2 (Millipore Sigma AB9610) O4 (Mouse hybridoma) MBP (AbD Serotec MCA409S) PDGFRa (BD Bioscience 556001) GFAP (ThermoFisher 13-0300) TUJ1 (Biolegend 801213) MAP2 (Sigma M9942) Caspase-3 (Cell Signaling 9664S) Click-iT™ EdU Cell Proliferation Kit (ThermoFisher C10337) Secondary antibodies of appropriate species were purchased from Jackson ImmunoResearch and Fisher Scientific.

    Techniques: Concentration Assay, Staining